Chimica | BIOINORGANIC CHEMISTRY

cod. 0522300003

BIOINORGANIC CHEMISTRY

	0522300003
	DIPARTIMENTO DI CHIMICA E BIOLOGIA "ADOLFO ZAMBELLI"

	CHEMISTRY
	2015/2016

PERCORSO COMUNE

	YEAR OF DIDACTIC SYSTEM 2010
	SECONDO SEMESTRE

TEACHING UNITS

	SSD	CFU	HOURS	ACTIVITY	TYPE OF ACTIVITY
	CHIM/03	6	48	LESSONS	SUPPLEMENTARY COMPULSORY SUBJECTS

	Objectives
	THE COURSE WILL INTRODUCE THE STUDENT TO THE KNOWLEDGE OF THE NEED AND THE AVAILABILITY OF INORGANIC ELEMENTS IN LIVING ORGANISMS. IT WILL EXAMINE THE DIFFERENT TYPES OF COMPLEXES BETWEEN METALS AND BIOLOGICAL MOLECULES AND MECHANISMS OF ACTION OF BIOLOGICAL PROCESSES INVOLVING INORGANIC ELEMENTS. FINALLY, THE STUDENT WILL BE INTRODUCED TO MORE ADVANCED RESEARCH TOPICS IN THE FIELD OF BIOINORGANIC, EG THE USE OF METALLIC COMPLEXES COMPOUNDS CONJUGATED WITH POLYMERS USED IN DIAGNOSTICS AND THERAPY.

THE COURSE WILL INTRODUCE THE STUDENT TO THE KNOWLEDGE OF THE NEED AND THE AVAILABILITY OF INORGANIC ELEMENTS IN LIVING ORGANISMS. IT WILL EXAMINE THE DIFFERENT TYPES OF COMPLEXES BETWEEN METALS AND BIOLOGICAL MOLECULES AND MECHANISMS OF ACTION OF BIOLOGICAL PROCESSES INVOLVING INORGANIC ELEMENTS. FINALLY, THE STUDENT WILL BE INTRODUCED TO MORE ADVANCED RESEARCH TOPICS IN THE FIELD OF BIOINORGANIC, EG THE USE OF METALLIC COMPLEXES COMPOUNDS CONJUGATED WITH POLYMERS USED IN DIAGNOSTICS AND THERAPY.

	Prerequisites
	NO PREREQUISITES

	Contents
	OVERVIEW OF BIOINORGANIC CHEMISTRY. WHAT IS BIOINORGANIC CHEMISTRY? PRINCIPLE OF COORDINATION CHEMISTRY RELATED TO BIOINORGANIC CHEMISTRY. THE CHELATE EFFECT , PKA VALUES OF COORDINATED LIGANDS, LIGAND EXCHANGE RATES, ELECTRONIC AND GEOMETRIC STRUCTURES OF METAL IONS IN BIOLOGY. PROPERTIES OF BIOLOGICAL MOLECULES. PROTEINS AND THEIR COSTITUENTS. PROTEINS AS LIGANDS. THE KINETIC ANALYSIS OF ENZYME REACTIONS. NUCLEIC ACIDS AND THEIR COSTITUENTS. RNA, DNA AND THEIR COSTITUENTS BLOCKS. METAL BINDING AND NUCLEIC ACID STRUCTURE. PROSTHETIC GROUPS. UPTAKE OF METAL CONTAINING UNITS IN BIOLOGY. BIOAVAILABILITY OF METAL IONS. ENRICHMENT STRATEGIES AND INTRACELLULAR CHEMISTRY OF METALS. SPONTANEOUS SELF-ASSEMBLY OF METAL CLUSTERS. CONTROL AND UTILIZATION OF METAL ION CONCENTRATION IN CELLS. BENEFICIAL AND TOXIC EFFECTS OF METAL IONS. AN EXAMPLE OF A BENEFICIAL METAL: IRON; AN EXAMPLE OF A TOXIC METAL: MERCURY. THE GENERATION AND USES OF METAL IONS CONCENTRATION GRADIENTS. METAL ION FOLDING AND CROSS-LINKING OF BIOMOLECULES. METAL ION STABILIZATION OF PROTEIN AND NUCLEIC ACID STRUCTURE. PROTEIN BINDING TO METALLATED DNA: MECHANISM OF ACTION OF FEW ANTICANCER AGENTS. BINDING OF METAL IONS AND COMPLEXES TO BIOMOLECULE-ACTIVE CENTERS. SELECTION AND INSERTION OF METAL IONS FOR PROTEIN SITES. PRESERVATION OF ELECTRONEUTRALITY. METAL-ION AND METAL-COMPLEX BINDING TO NUCLEIC ACID. BIOPOLYMER PROMOTES METAL-LIGAND INTERACTIONS. METAL FUNCTIONS IN METALLOPROTEINS. ELECTRON-TRANSFER PROTEINS. SUBSTRATE BINDING AND ACTIVATION BY NONREDOX MECHANISMS. ATOM- AND GROUP-TRANSFER CHEMISTRY. PROTEIN TUNING OF METAL PROPERTIES TO ACHIEVE SPECIFIC FUNCTIONS. METALS IN MEDICINE. METAL COMPLEXES IN DIAGNOSIS AND CANCER THERAPY. SYNTHETIC POLYMERS AND BIOPOLYMERS AS CARRIERS OF METAL COMPLEXES: THE EPR EFFECT.

Contents

OVERVIEW OF BIOINORGANIC CHEMISTRY. WHAT IS BIOINORGANIC CHEMISTRY?
PRINCIPLE OF COORDINATION CHEMISTRY RELATED TO BIOINORGANIC CHEMISTRY. THE CHELATE EFFECT , PKA VALUES OF COORDINATED LIGANDS, LIGAND EXCHANGE RATES, ELECTRONIC AND GEOMETRIC STRUCTURES OF METAL IONS IN BIOLOGY.
PROPERTIES OF BIOLOGICAL MOLECULES. PROTEINS AND THEIR COSTITUENTS. PROTEINS AS LIGANDS. THE KINETIC ANALYSIS OF ENZYME REACTIONS. NUCLEIC ACIDS AND THEIR COSTITUENTS. RNA, DNA AND THEIR COSTITUENTS BLOCKS. METAL BINDING AND NUCLEIC ACID STRUCTURE. PROSTHETIC GROUPS.

UPTAKE OF METAL CONTAINING UNITS IN BIOLOGY. BIOAVAILABILITY OF METAL IONS. ENRICHMENT STRATEGIES AND INTRACELLULAR CHEMISTRY OF METALS. SPONTANEOUS SELF-ASSEMBLY OF METAL CLUSTERS.
CONTROL AND UTILIZATION OF METAL ION CONCENTRATION IN CELLS. BENEFICIAL AND TOXIC EFFECTS OF METAL IONS. AN EXAMPLE OF A BENEFICIAL METAL: IRON; AN EXAMPLE OF A TOXIC METAL: MERCURY. THE GENERATION AND USES OF METAL IONS CONCENTRATION GRADIENTS.

METAL ION FOLDING AND CROSS-LINKING OF BIOMOLECULES. METAL ION STABILIZATION OF PROTEIN AND NUCLEIC ACID STRUCTURE. PROTEIN BINDING TO METALLATED DNA: MECHANISM OF ACTION OF FEW ANTICANCER AGENTS.

BINDING OF METAL IONS AND COMPLEXES TO BIOMOLECULE-ACTIVE CENTERS. SELECTION AND INSERTION OF METAL IONS FOR PROTEIN SITES. PRESERVATION OF ELECTRONEUTRALITY. METAL-ION AND METAL-COMPLEX BINDING TO NUCLEIC ACID. BIOPOLYMER PROMOTES METAL-LIGAND INTERACTIONS.

METAL FUNCTIONS IN METALLOPROTEINS. ELECTRON-TRANSFER PROTEINS. SUBSTRATE BINDING AND ACTIVATION BY NONREDOX MECHANISMS. ATOM- AND GROUP-TRANSFER CHEMISTRY. PROTEIN TUNING OF METAL PROPERTIES TO ACHIEVE SPECIFIC FUNCTIONS.

METALS IN MEDICINE. METAL COMPLEXES IN DIAGNOSIS AND CANCER THERAPY. SYNTHETIC POLYMERS AND BIOPOLYMERS AS CARRIERS OF METAL COMPLEXES: THE EPR EFFECT.

	Teaching Methods
	FRONTAL LECTURES

	Verification of learning
	ORAL EXAM (VIVA VOCE EXAM)

	Texts
	- HAND OUTS - S.J. LIPPARD, J.M. BERG “PRINCIPLES OF BIOINORGANIC CHEMISTRY” - UNIVERSITY SCIENCE BOOKS. - W. KAIM, B. SCHWEDERSKI “BIOINORGANIC CHEMISTRY: INORGANIC ELEMENTS IN THE CHEMISTRY OF LIFE” - WILEY