Chimica | BIOORGANIC CHEMISTRY

cod. 0522300046

BIOORGANIC CHEMISTRY

	0522300046
	DEPARTMENT OF CHEMISTRY AND BIOLOGY "ADOLFO ZAMBELLI"
	EQF7
	CHEMISTRY
	2018/2019

CURRICULUM BIOMOLECULAR CHEMISTRY Cohort 2017/2018

	YEAR OF COURSE 2
	YEAR OF DIDACTIC SYSTEM 2016
	SECONDO SEMESTRE

TEACHING UNITS

	SSD	CFU	HOURS	ACTIVITY	TYPE OF ACTIVITY
	CHIM/06	6	48	LESSONS	SUPPLEMENTARY COMPULSORY SUBJECTS

	Objectives
	THE COURSE INTENDS TO PROVIDE CHEMICAL AND BIOMOLECULAR KNOWLEDGE OF AMINO ACIDS AND PROTEINS, NUCLEIC ACIDS, CARBOHYDRATES, LIPIDS USING STRATEGIES AND TECHNIQUES ASSOCIATED WITH ORGANIC CHEMISTRY. THE MAIN KNOWLEDGE ACQUIRED BY THE STUDENTS WILL REGARD TO: - CHEMICAL PROPERTIES AND REACTIVITY OF THE MAIN BIOMOLECULES AND BIOMACROMOLECULES CLASSES OF COMPOUNDS. - MAIN INSTRUMENTAL METHODS FOR THE ANALYSIS OF THESE CLASSES OF COMPOUNDS. - CHEMICAL SYNTHESIS METHODS AND AUTOMATED PROCEDURES. - PROPERTIES OF ANALOGUES AND APPLICATIONS IN THE PHARMACEUTICAL FIELD. THE MAIN SKILLS DEVELOPED BY THE STUDENTS WILL BE: - ADDRESSING TO ANALYTICAL PROBLEMS OF COMPLEX MIXTURES OF BIOMOLECULES AND BIOMACROMOLECULES. - RESOLVING PROBLEMS RELATED TO THE SYNTHESIS AND TO THE PURIFICATION OF BIOMACROMOLECULES. - CRITICAL ANALYSIS OF SCIENTIFIC LITERATURE IN THE FIELD.

THE COURSE INTENDS TO PROVIDE CHEMICAL AND BIOMOLECULAR KNOWLEDGE OF AMINO ACIDS AND PROTEINS, NUCLEIC ACIDS, CARBOHYDRATES, LIPIDS USING STRATEGIES AND TECHNIQUES ASSOCIATED WITH ORGANIC CHEMISTRY.

THE MAIN KNOWLEDGE ACQUIRED BY THE STUDENTS WILL REGARD TO:

- CHEMICAL PROPERTIES AND REACTIVITY OF THE MAIN BIOMOLECULES AND BIOMACROMOLECULES CLASSES OF COMPOUNDS.
- MAIN INSTRUMENTAL METHODS FOR THE ANALYSIS OF THESE CLASSES OF COMPOUNDS.
- CHEMICAL SYNTHESIS METHODS AND AUTOMATED PROCEDURES.
- PROPERTIES OF ANALOGUES AND APPLICATIONS IN THE PHARMACEUTICAL FIELD.

THE MAIN SKILLS DEVELOPED BY THE STUDENTS WILL BE:

- ADDRESSING TO ANALYTICAL PROBLEMS OF COMPLEX MIXTURES OF BIOMOLECULES AND BIOMACROMOLECULES.
- RESOLVING PROBLEMS RELATED TO THE SYNTHESIS AND TO THE PURIFICATION OF BIOMACROMOLECULES.
- CRITICAL ANALYSIS OF SCIENTIFIC LITERATURE IN THE FIELD.

	Prerequisites
	BASIC ORGANIC AND BIOCHEMISTRY

	Contents
	: - CHEMICAL STRUCTURE, REACTIVITY AND BIOLOGICAL ACTIVITY OF AMINO ACIDS, PEPTIDES AND PROTEINS. RACEMIC AND ASYMMETRIC SYNTHESIS OF NATURAL AND NON NATURAL AMINO ACIDS. PEPTIDE SYNTHESIS. FUNCTION OF THE PROTEINS. MONOCLONAL ANTIBODIES (MAB). BIOCONJUGATES IN DRUG DESIGN. CHEMICAL MODIFICATION OF PROTEINS. PEPTIDOMIMETIC. ANALYSIS OF AMINO ACIDS AND PROTEINS. EDMAN AND SANGER METHODOLOGIES. - NUCLEIC ACIDS. NUCLEOSIDES. MONO AND POLYNUCLEOTIDES. HETEROCYCLIC PURINES AND PYRIMIDINES. BASES OF DNA. THE MODEL OF WATSON-CRICK. DUPLEX, TRIPLEX AND G-QUADRUPLEX. ANTISENSE NUCLEOTIDES: THE CASE OF NUSINERSEN FOR THE TREATMENT OF SMA. CHEMICAL SYNTHESIS OF NUCLEOSIDES AND OLIGONUCLEOTIDES. NUCLEOSIDES OF PHARMACEUTICAL INTEREST: ANTIVIRALS. MECHANISMS OF DAMAGE AND REPAIR OF DNA. DNA AS A TARGET FOR THERAPEUTIC AGENTS: ANTICANCER. SANGER DNA SEQUENCING METHODOLOGY. - MONO-, OLIGO- AND POLYSACCHARIDES: CHEMISTRY, FUNCTIONS, PROPERTIES AND APPLICATIONS. COMPLEXITY OF POLYSACCHARIDES: FORMATION OF GLYCOSIDIC BOND. TECHNIQUES FOR SYNTHESIS, PURIFICATION AND CHARACTERIZATION. MODIFICATIONS OF MONO-, OLIGO- AND POLYSACCHARIDES. GLYCOCONJUGATES. DRUG DELIVERY SYSTEMS. GLYCOSAMINOGLYCANS: HYALURONIC ACID. - LIPIDS: CLASSIFICATION OF LIPIDS. CHEMICAL PROPERTIES. TRIGLYCERIDES, PHOSPHOLIPIDS, SPHINGOLIPIDS, GLYCOLIPIDS, LIPOPROTEIN. LIPOSOMES AND MICELLES.

Contents

: - CHEMICAL STRUCTURE, REACTIVITY AND BIOLOGICAL ACTIVITY OF AMINO ACIDS, PEPTIDES AND PROTEINS. RACEMIC AND ASYMMETRIC SYNTHESIS OF NATURAL AND NON NATURAL AMINO ACIDS. PEPTIDE SYNTHESIS. FUNCTION OF THE PROTEINS. MONOCLONAL ANTIBODIES (MAB). BIOCONJUGATES IN DRUG DESIGN. CHEMICAL MODIFICATION OF PROTEINS. PEPTIDOMIMETIC. ANALYSIS OF AMINO ACIDS AND PROTEINS. EDMAN AND SANGER METHODOLOGIES.
- NUCLEIC ACIDS. NUCLEOSIDES. MONO AND POLYNUCLEOTIDES. HETEROCYCLIC PURINES AND PYRIMIDINES. BASES OF DNA. THE MODEL OF WATSON-CRICK. DUPLEX, TRIPLEX AND G-QUADRUPLEX. ANTISENSE NUCLEOTIDES: THE CASE OF NUSINERSEN FOR THE TREATMENT OF SMA. CHEMICAL SYNTHESIS OF NUCLEOSIDES AND OLIGONUCLEOTIDES. NUCLEOSIDES OF PHARMACEUTICAL INTEREST: ANTIVIRALS. MECHANISMS OF DAMAGE AND REPAIR OF DNA. DNA AS A TARGET FOR THERAPEUTIC AGENTS: ANTICANCER. SANGER DNA SEQUENCING METHODOLOGY.

- MONO-, OLIGO- AND POLYSACCHARIDES: CHEMISTRY, FUNCTIONS, PROPERTIES AND APPLICATIONS. COMPLEXITY OF POLYSACCHARIDES: FORMATION OF GLYCOSIDIC BOND. TECHNIQUES FOR SYNTHESIS, PURIFICATION AND CHARACTERIZATION. MODIFICATIONS OF MONO-, OLIGO- AND POLYSACCHARIDES. GLYCOCONJUGATES. DRUG DELIVERY SYSTEMS. GLYCOSAMINOGLYCANS: HYALURONIC ACID.
- LIPIDS: CLASSIFICATION OF LIPIDS. CHEMICAL PROPERTIES. TRIGLYCERIDES, PHOSPHOLIPIDS, SPHINGOLIPIDS, GLYCOLIPIDS, LIPOPROTEIN. LIPOSOMES AND MICELLES.

	Teaching Methods
	THE COURSE CONTEMPLATES LECTURES WITH SUPPORT OF ELECTRONIC PRESENTATIONS AND THE SOLVING OF RELEVANT PROBLEMS ON THE BLACKBOARD. ALL TEACHING MATERIALS WILL BE MADE AVAILABLE TO STUDENTS AT THE END OF EACH LESSON AS FILES IN PDF FORMAT.

	Verification of learning
	THE EXAM OCCURS EXCLUSIVELY THROUGH AN ORAL INTERVIEW ON THE THEORETICAL AND METHODOLOGICAL CONTENTS AS INDICATED IN THE COURSE PROGRAM. THE AIM IS TO ESTABLISH THE LEVEL OF KNOWLEDGE AND CAPACITY OF UNDERSTANDING REACHED BY THE STUDENT, AND TO VERIFY THE ABILITY OF THE PRESENTATION USING THE APPROPRIATE TERMINOLOGY.

	Texts
	- BIOORGANIC CHEMISTRY, EDITED BY S. M. HECHT, OXFORD UNIVERSITY PRESS, 1996. - SELECTED TOPICS FROM REVIEWS ON BIOCONJUGATION. - CHIMICA ORGANICA, J. MCMURRY, PICCIN ALTERNATIVELY: - CHEMICAL APPROACHES TO THE SYNTHESIS OF PEPTIDES AND PROTEINS, EDITED BY P. LLOYD-WILLIAMS, F. ALBERICIO, E. GIRAT, CRC PRESS LLC . - CARBOHYDRATES IN CHEMISTRY AND BIOLOGY, EDITED BY B. ERNST, G. W. HART, P. SINAY, WILEY-VCH VERLAG GMBH, 2000. - METHODS IN MEMBRANE LIPIDS, EDITED BY A. M. DOPICO, SPRINGER SCIENCE & BUSINESS MEDIA, 2007

Texts

- BIOORGANIC CHEMISTRY, EDITED BY S. M. HECHT, OXFORD UNIVERSITY PRESS, 1996.
- SELECTED TOPICS FROM REVIEWS ON BIOCONJUGATION.
- CHIMICA ORGANICA, J. MCMURRY, PICCIN

ALTERNATIVELY:
- CHEMICAL APPROACHES TO THE SYNTHESIS OF PEPTIDES AND PROTEINS, EDITED BY P. LLOYD-WILLIAMS, F. ALBERICIO, E. GIRAT, CRC PRESS LLC .

- CARBOHYDRATES IN CHEMISTRY AND BIOLOGY, EDITED BY B. ERNST, G. W. HART, P. SINAY, WILEY-VCH VERLAG GMBH, 2000.

- METHODS IN MEMBRANE LIPIDS, EDITED BY A. M. DOPICO, SPRINGER SCIENCE & BUSINESS MEDIA, 2007