Biomacromolecules

Chimica Biomacromolecules

0522300032
DEPARTMENT OF CHEMISTRY AND BIOLOGY "ADOLFO ZAMBELLI"
EQF7
CHEMISTRY
2022/2023

YEAR OF COURSE 2
YEAR OF DIDACTIC SYSTEM 2016
SPRING SEMESTER
CFUHOURSACTIVITY
540LESSONS
112EXERCISES
ExamDate
CHIMICA DELLE BIOMACROMOLECOLE02/03/2023 - 10:00
Objectives
THE COURSE INTENDS TO PROVIDE CHEMICAL AND BIOMOLECULAR KNOWLEDGE OF AMINO ACIDS AND PROTEINS, NUCLEIC ACIDS, CARBOHYDRATES, LIPIDS USING STRATEGIES AND TECHNIQUES ASSOCIATED WITH ORGANIC CHEMISTRY.

THE MAIN KNOWLEDGE ACQUIRED BY THE STUDENTS WILL REGARD TO:

- CHEMICAL PROPERTIES AND REACTIVITY OF THE MAIN BIOMOLECULES AND BIOMACROMOLECULES CLASSES OF COMPOUNDS.
- MAIN INSTRUMENTAL METHODS FOR THE ANALYSIS OF THESE CLASSES OF COMPOUNDS.
- CHEMICAL SYNTHESIS METHODS AND AUTOMATED PROCEDURES.
- PROPERTIES OF ANALOGUES AND APPLICATIONS IN THE PHARMACEUTICAL FIELD.


THE MAIN SKILLS DEVELOPED BY THE STUDENTS WILL BE:

- ADDRESSING TO ANALYTICAL PROBLEMS OF COMPLEX MIXTURES OF BIOMOLECULES AND BIOMACROMOLECULES.
- RESOLVING PROBLEMS RELATED TO THE SYNTHESIS AND TO THE PURIFICATION OF BIOMACROMOLECULES.
- CRITICAL ANALYSIS OF SCIENTIFIC LITERATURE IN THE FIELD.
Prerequisites
BASIC ORGANIC AND BIOCHEMISTRY
Contents
: - CHEMICAL STRUCTURE, REACTIVITY AND BIOLOGICAL ACTIVITY OF AMINO ACIDS, PEPTIDES AND PROTEINS. RACEMIC AND ASYMMETRIC SYNTHESIS OF NATURAL AND NON NATURAL AMINO ACIDS. PEPTIDE SYNTHESIS. FUNCTION OF THE PROTEINS. MONOCLONAL ANTIBODIES (MAB). BIOCONJUGATES IN DRUG DESIGN. CHEMICAL MODIFICATION OF PROTEINS. PEPTIDOMIMETIC. ANALYSIS OF AMINO ACIDS AND PROTEINS. EDMAN AND SANGER METHODOLOGIES.
- NUCLEIC ACIDS. NUCLEOSIDES. MONO AND POLYNUCLEOTIDES. HETEROCYCLIC PURINES AND PYRIMIDINES. BASES OF DNA. THE MODEL OF WATSON-CRICK. DUPLEX, TRIPLEX AND G-QUADRUPLEX. ANTISENSE NUCLEOTIDES: THE CASE OF NUSINERSEN FOR THE TREATMENT OF SMA. CHEMICAL SYNTHESIS OF NUCLEOSIDES AND OLIGONUCLEOTIDES. NUCLEOSIDES OF PHARMACEUTICAL INTEREST: ANTIVIRALS. MECHANISMS OF DAMAGE AND REPAIR OF DNA. DNA AS A TARGET FOR THERAPEUTIC AGENTS: ANTICANCER. SANGER DNA SEQUENCING METHODOLOGY.

- MONO-, OLIGO- AND POLYSACCHARIDES: CHEMISTRY, FUNCTIONS, PROPERTIES AND APPLICATIONS. COMPLEXITY OF POLYSACCHARIDES: FORMATION OF GLYCOSIDIC BOND. TECHNIQUES FOR SYNTHESIS, PURIFICATION AND CHARACTERIZATION. MODIFICATIONS OF MONO-, OLIGO- AND POLYSACCHARIDES. GLYCOCONJUGATES. DRUG DELIVERY SYSTEMS. GLYCOSAMINOGLYCANS: HYALURONIC ACID.
- LIPIDS: CLASSIFICATION OF LIPIDS. CHEMICAL PROPERTIES. TRIGLYCERIDES, PHOSPHOLIPIDS, SPHINGOLIPIDS, GLYCOLIPIDS, LIPOPROTEIN. LIPOSOMES AND MICELLES.

EXPERIENCES WILL BE CARRIED OUT ON THE CHARACTERIZATION AND ANALYSIS OF BIOMOLECULES, USING NUCLEAR MAGNETIC RESONANCE TECHNIQUES (1H, 13C AND 31P NMR) AND GAS CHROMATOGRAPHY (ANALYSIS OF FATTY ACID MIXTURES).
Teaching Methods
THE COURSE CONTEMPLATES LECTURES WITH SUPPORT OF ELECTRONIC PRESENTATIONS AND THE SOLVING OF RELEVANT PROBLEMS ON THE BLACKBOARD. ALL TEACHING MATERIALS WILL BE MADE AVAILABLE TO STUDENTS AT THE END OF EACH LESSON AS FILES IN PDF FORMAT.
Verification of learning
THE EXAM OCCURS EXCLUSIVELY THROUGH AN ORAL INTERVIEW ON THE
THEORETICAL AND METHODOLOGICAL CONTENTS AS
INDICATED IN THE COURSE PROGRAM. THE AIM IS TO ESTABLISH THE LEVEL OF
KNOWLEDGE AND CAPACITY OF
UNDERSTANDING REACHED BY THE STUDENT, AND TO VERIFY THE ABILITY OF
THE PRESENTATION USING THE
APPROPRIATE TERMINOLOGY.
Texts
- BIOORGANIC CHEMISTRY, EDITED BY S. M. HECHT, OXFORD UNIVERSITY PRESS, 1996.
- SELECTED TOPICS FROM REVIEWS ON BIOCONJUGATION.
- CHIMICA ORGANICA, J. MCMURRY, PICCIN

ALTERNATIVELY:
- CHEMICAL APPROACHES TO THE SYNTHESIS OF PEPTIDES AND PROTEINS, EDITED BY P. LLOYD-WILLIAMS, F. ALBERICIO, E. GIRAT, CRC PRESS LLC .

- CARBOHYDRATES IN CHEMISTRY AND BIOLOGY, EDITED BY B. ERNST, G. W. HART, P. SINAY, WILEY-VCH VERLAG GMBH, 2000.

- METHODS IN MEMBRANE LIPIDS, EDITED BY A. M. DOPICO, SPRINGER SCIENCE & BUSINESS MEDIA, 2007
More Information
FOR FURTHER INFORMATION: AMASSA@UNISA.IT
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