PLANT GENETICS AND BREEDING

Gestione e Valorizzazione delle Risorse Agrarie e delle Aree Protette PLANT GENETICS AND BREEDING

0712200010
DIPARTIMENTO DI FARMACIA
EQF6
GESTIONE E VALORIZZAZIONE DELLE RISORSE AGRARIE E DELLE AREE PROTETTE
2022/2023

OBBLIGATORIO
YEAR OF COURSE 2
YEAR OF DIDACTIC SYSTEM 2016
SPRING SEMESTER
CFUHOURSACTIVITY
963LESSONS
Objectives
THE MAIN OBJECTIVES OF THE COURSE IS TO GIVE TOMTHE STUDENTS THE ABILITIES TO LEARN AND UNDERSTAND:
•BASIC ELEMENTS ON NUCLEIC ACIDS AND TRANSMISSIBILITY OF GENETIC TRAITS;
•GENETIC AND MOLECULAR ANALYSIS OF PLANT GENOME AND REGULATION OF GENE EXPRESSION, IN RESPONSE TO ENDOGENOUS AND ENRINONMENTAL SIGNALS;
•STANDARD AND INNOVATIVE METHODOLOGIES AND TECNOLOGIES FOR IMPROVING QUALI-QUANTITATIVE TRAITS IN CROPS.

AT THE END OF THE COURSE, THE STUDENTS WILL BE ABLE TO APPLY HIS/HER ACQUIRED SKILLS TO:
•ANALYZE THE GENETIC DETERMINANTS, AND THEIR TRANSMISSIBILITY, CONTROLLING THE MAIN YIELD COMPONENTS AND QUALITY OF PLANT PRODUCTS;
•EVALUATE THE EFFECTS OF PLANT GENOME –ENVIRONMENT INTERACTION, TO OPTIMIZE THE AGRONOMIC PARAMETERS AND SUSTAINABILITY OF CROP PLANTS;
•IDENTIFY AND IMPLEMENT THE MOST APPROPRIATE PROCEDURES TO THE RELEASE OF NEW CROP VARIETIES WITH THE FINAL AIM TO INCREASE PRODUCTIVITY AND QUALITY OF THE MAIN CROP PLANTS, INTEGRATING CLASSICAL TECHNIQUES IN PLANT BREEDING AND BIOTECHNOLOGICAL TECHNOLOGIES.
Prerequisites
TO MAXIMIZE COMPREHENSION OF THE MAJORITY OF THE TOPICS INCLUDED IN THE PROGRAM, THE STUDENTS ARE REQUESTED TO PASS THE EXAMS OF THE COURSE IN BIOLOGY AND BOTANY (1ST YEAR).
Contents
PART I - ELEMENTS OF FORMAL GENETICS
- 0.5 CREDIT – NUCLEIC ACIDS: CHEMICAL AND PHYSICAL STRUCTURE. PROKARYOTIC AND EUKARYOTYC CHROMOSOMES, DNA REPLICATION
- 1.5 CREDITS – THE GENETIC FLOW OF INFORMATION: GENE TRANSCRIPTION AND TRANSLATION, CONTROL OF GENE EXPRESSION TO ENDOGENOUS (PHYTOHORMONES, MAINLY) AND EXOGENOUS SIGNALS (LIGHT, PHOTOPERIOD, WATER AVAILABILITY, TEMPERATURE);
- 1.0 CREDIT- HEREDITABILITY AND TRANSMISSIBILITY OF GENETIC TRAITS; MENDELIAN GENETICS, HYBRIDIZATION, LINKED GENES;
- 1.0 CREDIT- GENETIC VARIABILITY: RECOMBINATION, MUTATIONS, GENOTYPE-ENVIROMENT INTERACTION, HEREDITABILITY OF QUANTITATIVE TRAITS;
- 0.5 CREDIT PRACTISING GENETIC QUIZZES ON MENDELIAN GENETICS, RECOMBINATION, LINKED GENES
- 1.0 CREDIT PRACTICE LAB: DNA EXTRACTION AND ANALYSIS (THREE GROUPS, MAXIMUM 35 STUDENTS EACH GROUP).
- 0.5 CREDIT- FIELD PRACTICE- CROSSES AND TRANSMISSIBILITY OF GENETIC TRAITS (OPEN FIELD AND GREENHOUSE)

PART II PLANT BREEDING
-1.5 CREDITS- GENETICS OF PLANT REPRODUCTION: GENETIC TRAITS CONTROLLING AUTOGAMY, HETEROGAMY /MALE-STERILITY AND SELF-INCOMPATIBILITY),. INBREEDING AND HETEROSIS
-1.5 CREDITS- SELECTION AND REALESE OF NEW CROP VARIETIES: MAIN TECHNIQUES AND PROCEDURES FOR OBTAINIG PURE LINES, HYBRIDS, MOLECULAR MARKERS AND MARKER ASSISTED SELECTION, TECHNIQUES OF RECOMBIANT DNA APPLIED TO VARIETAL TRACEABILITY AND TO PRODUCION OF IMPROVED TRANSGENIC PLANTS
1.0 CREDIT- PRACTICE LAB: MOLECULAR MARKERS BY POLYMERASE CHAIN REACTION, DNA ELECTROPHORESIS THREE GROUPS, MAXIMUM 35 STUDENTS).
Teaching Methods
DURING THE COURSE (9 CREDITS), FOR A TOTAL OF 63 HOURS, THE TOPICS INCLUDED IN THE PROGRAM, WILL BE PRESENTED AS:
-LECTURES (6 CREDITS, 42 HOURS), USING SLIDES, ANIMATIONS AND RESOURCES AVAILABLE ON-LINE;
-EXCERCISING CLASS (0, 5 CREDIT, 3,5 HOURS)- SOLUTIONS OF QUIZZES AND EXCERCISES ON MENDELIAN GENETICS. ADDITIONAL EXCERCISES AND QUIZZES WILL BE PROVIDED TO THE STUDENTS FOR PRACTISING AT HOME;
-PRACTICAL AND FIELD ACTIVITIES (2,5 CFU, 17,5 HOURS).
ATTENDANCE OF THE ABOVE DESCRIBED ACTIVITIES IS NOT MANDATORY, BUT STRONGLY SUGGESTED.
Verification of learning
ACQUIRED KNOWLEDGE AND SKILLS IN PLANT GENETICS AND BREEDING BY THE STUDENTS WILL BE EVALUATED THROUGH:
-A FIRST WRITTEN TEST, COMPREHENSIVE OF TEN OPEN OR MULTIPLE CHOICE QUESTIONS ON TOPICS INCLUDED IN PART I (60 MINUTES AVAILABLE, MAXIMUM SCORE 10 POINTS, MINIMUM SCORE 6 POINTS TO BE PASSED):
-A SECOND WRITTEN TEST, COMPREHENSIVE OF 5 PROBLEMS/QUIZZES ON MENDIALIAN GENETICS, RECOMBINATION AND LINKED GENES ((60 MINUTES AVAILABLE, MAXIMUM SCORE 5 POINTS, MINIMUM SCORE 3 POINTS TO PASS);
-A THIRD WRITTEN TEST, COMPREHENSIVE OF TEN OPEN OR MULTIPLE CHOICE QUESTIONS ON TOPICS INCLUDED IN PART II (60 MINUTES AVAILABLE, MAXIMUM SCORE 10 POINTS, MINIMUM SCORE 6 POINTS TO PASS);
-A WRITTEN REPORT ON THE PRACTICAL ACRIVITIES IN LABORATORY, OPEN FIELD OR GREENHOUSE, ACCORIDNG TO THE INDICATIONS GIVEN BY THE INSTRUCTOR (MAXIMUM SCORE 5 POINTS, MINIMUM SCORE 3 POINTS TO PASS).
THE FINAL SCORE IS GIVEN BY THE SUM OF POINTS FOR EACH OF THE ABOVE DESCRIBED TEST/REPORT:

FOR STUDENTS WHO HAVE NOT PERFORMED THE WRITTEN TESTS, OR WHO HAS REFUSED THE SCORE OBTAINED IN THE WRITTEN TESTS OR HAVE NOT PASSED THE WRITTEN TESTS, THE FINAL EVALUATION CONSISTS OF:
-A WRITTEN TEST, COMPREHENSIVE OF 5 PROBLEMS/QUIZZES ON MENDIALIAN GENETICS, RECOMBINATION AND LINKED GENES (60 MINUTES AVAILABLE, MAXIMUM SCORE 5 POINTS, MINIMUM SCORE 3 POINTS TO PASS). PASSING THIS TEST IS MANDATORY TO ACCESS TO THE ORAL INTERVIEW;
-AN ORAL INTERVIEW ON THE TOPICS OF THE PROGRAM, EXCEPT MENDELIAN GENETICS.

THE FINAL SCORE IS EXPRESSED IN 30/30. TO PASS IT IS NECESSARY TO HAVE A MINIMUM SCORE OF 18/30.
AN ADDITIONAL “CUM LADE” MAY BE ATTRIBUTED TO STUDENTS WITH THE MAXIMUM SCORE (30/30) WHO HAVE DEMONSTRATED THEIR AUTONOMY IN DISCUSSING AND APPLYING THE ACQUIRE KNOWLEDGE AND SKILLS IN PLANT GENETICS AND BREEDING PROGRAMS.
Texts
REFERENCE TEXT-BOOK
RUSSELL P.J., WOLFE S.L., HERTZ P.E., STARR C., MCMILLIAN B. (2016) GENETICA AGRARIA, EDISES



ADDITIONAL MATERIAL
CHAPTERS ON TOPICS OF THE PROGRAM POORLY DEVELOPED OR NOT INCLUDED IN THE REFERENCE TEXT BOOK WILL BE AVAILABLE FOR THE STUDENTS, TOGETHER WITH EXTRA EXERCISES ON MENDELIAN GENETICS FOR PRACTICING AT HOME , AND AVAILABLE FROM A SHARED ON-LINE FOLDER, generated in the Microsoft Teams virtual class dedicated to the course

OTHER COMPELLING TEXT-BOOK THAT THE STUDENTS MIGHT CONSULT:
GRIFFITH A.J., WESSLER S.R., CARROLL S.B., DOEBLEY J., 2013- GENETICA, ZANICHELLI
G. BARCACCIA, M. FALCINELLI (2008) - GENETICA E GENOMICA VOL. 2 MIGLIORAMENTO GENETICO. LIGUORI EDITOR
More Information
PLEASE, CONTACT THE INSTRUCTOR FOR FURTHER INFORMATION BY EMAIL:
ALEONE@UNISA.IT
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