C.I. BIOCHIMICA E BIOLOGIA

Dentistry C.I. BIOCHIMICA E BIOLOGIA

1060200011
DIPARTIMENTO DI MEDICINA, CHIRURGIA E ODONTOIATRIA "SCUOLA MEDICA SALERNITANA"
EQF7
DENTISTRY
2021/2022

OBBLIGATORIO
YEAR OF COURSE 2
YEAR OF DIDACTIC SYSTEM 2015
AUTUMN SEMESTER
CFUHOURSACTIVITY
1C.I. BIOCHIMICA E BIOLOGIA - MOD. BIOCHIMICA
333LESSONS
2C.I. BIOCHIMICA E BIOLOGIA - MOD. BIOLOGIA MOLECOLARE
777LESSONS
3C.I. BIOCHIMICA E BIOLOGIA - MOD. SCIENZE E TECNICHE DI MEDICINA DI LABORATORIO
333LESSONS
Objectives
THIS COURSE IS DESIGNED TO PROVIDE STUDENTS WITH: FUNDAMENTALS OF BIOMOLECULES STRUCTURES AND FUNCTIONS. FUNDAMENTALS OF MAIN METABOLIC PATHWAYS
AT THE END OF THE COURSE THE STUDENT WILL ALSO LEARN THE FUNDAMENTAL MOLECULAR MECHANISMS RELATED TO THE PROPAGATION AND MAINTENANCE OF GENETIC INFORMATION; WILL KNOW THE MOLECULAR MECHANISMS OF GENE EXPRESSION AND ITS REGULATIONS; WILL KNOW THE GENERAL PROCESSES THROUGH WHICH GENETIC INFORMATION IS TRANSMITTED AND EXPRESSED. WILL BE ABLE TO DESCRIBE AND SELECT APPROPRIATE TECHNOLOGIES FOR THE ANALYSIS OF BIOMOLECULAR MECHANISMS AND THE IDENTIFICATION OF MOLECULAR ORIGIN OF THE DISEASE.
AT THE END OF THE COURSE THE STUDENT WILL HAVE THE ABILITY TO APPLY CORRECTLY THE METHODS DESIGNED TO DETECT CLINICAL FINDING, AND LABORATORY TEST, USING CRITICAL INTERPRETATION AND PATHOPHYSIOLOGICAL CONSIDERATIONS. THEY MUST HAVE ALSO THE CAPACITY TO EVALUATE THE RELATIONSHIP COST / BENEFIT IN THE CHOICE OF DIAGNOSTIC PROCEDURES, TAKING NOTE OF THE PRINCIPLES OF EVIDENCE-BASED MEDICINE.

IN AGREEMENT WITH THE DUBLIN DESCRIPTORS, AT THE END OF THE COURSE THE STUDENT WILL DEMONSTRATE:
1.HAVE REACHED A BASIC KNOWLEDGE AND UNDERSTANDING OF BIOCHEMISTRY THAT ALLOWS HIM TO SET UP THE ANALYSIS OF THE CORRELATIONS BETWEEN THE STRUCTURE OF BIOMOLECULES AND THEIR FUNCTIONS IN PHYSIOLOGICAL AND PATHOLOGICAL CONDITIONS;
2.ABILITY TO ANALYZE, DISCUSS AND PERFORM SIMPLE CORRELATIONS OF DATA OR ASSUMPTIONS;
3.BEING ABLE EVALUATE INDEPENDENTLY ANY DIFFERENT OPINIONS EXIST IN CERTAIN TOPICS TREATED IN THE COURSE;
4.BEING ABLE TO PROPERLY EXPOSE IN ITALIAN AND WITH PROPERTIES OF LANGUAGE KNOWLEDGE ACQUIRED;
5.KNOWING HOW TO COMMUNICATE WITH THEIR PEERS IN A CRITICAL MANNER THE THEMES STUDIED AND TO SUPPORT INTERDISCIPLINARY ARGUMENTS.
Prerequisites
IN ORDER TO UNDERSTAND AND KNOW HOW TO APPLY THE TOPICS DISCUSSED IN TO THE COURSE STUDENTS MUST HAVE PASSED THE CHEMISTRY AND PROPEDEUTIC BIOCHEMISTRY AND APPLIED BIOLOGY
Contents
THE COURSE IS SUPPLEMENTED BY THREE MODULES: MODULE A BIOCHEMISTRY (3 CFU) MODULE B MOLECULAR BIOLOGY (7CFU) MODULE C LABORATORY MEDICINE SCIENCES AND TECHNIQUES OF LESSONS AND SUPPLEMENTARY TEACHING.
TOPICS COVERED IN THE MODULES:
MODULE A: AMINOACIDS: STRUCTURE, STEREOCHEMISTRY AND DERIVATIVES. PROTEINS: STRUCTURE AND EVOLUTION. MYOGLOBIN, HEMOGLOBIN, MYOSIN AND ACTIN, COLLAGEN ED ELASTIN. ANTIBODIES. CARBOHYDRATES: STRUCTURE AND DERIVATIVES OF SIMPLE AND COMPLEX SUGARS. LIPIDS: FATTY ACIDS, TAG, STEROIDS, ETC. NUCLEIC ACIDS: STRUCTURE, FUNCTION AND COMPOSITION OF NUCLEOTIDES, DNA, RNA. VITAMINS: STRUCTURE, FUNTIONS AND ENZYME CATALYSIS: KINETIC AND THERMODYNAMIC REMARKS. MICHAELIS AND ALLOSTERIC ENZYMES. INHIBITION AND REGULATION.METABOLIC PATHWAYS: THERMODYNAMICS AND METABOLIC CONTROL. ATP, NAD, FAD GLUCOSE CATABOLISM: AEROBIC AND ANAEROBIC GLYCOLYSIS, AND ITS CONTROL. PENTOSE PHOSPHATE PATHWAY. METABOLISM OF GLYCOGEN, GLUCONEOGENESIS, GLICAEMIA REGULATION. KREBS CYCLE: ACETYL-COA, ENZYMES, REGULATION AND RELATED REACTIONS. ELECTRON TRANSPORT CHAIN AND OXIDATIVE PHOSPHORILATION. ATP PRODUCTION, AEROBIC METABOLISM AND ITS IMPLICATIONS. LIPIDS METABOLISM: DIGESTION, LIPOPROTEINS, LIPID HOMEOSTASISFATTY ACIDS: BETA-OXIDATION, BIOSYNTHESIS AND METABOLIC CONTROL. KETON BODIES. CHOLESTEROL. METABOLISM OF AMINOACIDS AND PROTEINS. INTEGRATION E REGULATION OF ENERGETIC METABOLISM. ORGAN METABOLISM. DISORDERS OF METABOLISM: FASTING, DIABETES, OBESITY. METABOLISM OF NUCLEOTIDES: SYNTHESIS AND DEGRADATION. URIC ACID DESTINY.

MODULE B: STRUCTURE AND ORGANIZATION OF GENETIC MATERIAL IN PROKARYOTES, VIRUSES AND EUKARYOTES; STRUCTURE AND TOPOLOGY OF DNA; STRUCTURE OF RNA; CHROMATIN; HISTONES AND NUCLEOSOMES; REGULATION OF CHROMATIN STRUCTURE; EPIGENETICS.
REPLICATION AND REPAIR OF DNA DAMAGE: MECHANISM OF ACTION OF DNA POLYMERASES, STAGE OF START AND TERMINATION OF REPLICATION, TELOMERES AND TELOMERASE; REPLICATION ERRORS AND THEIR REPAIR; DIFFERENT TYPES OF MUTATIONS.
TRANSCRIPTION AND POST-TRANSCRIPTIONAL MODIFICATIONS, STRUCTURE AND FUNCTION OF RNA POLYMERASES, PROMOTERS OF RNA POLYMERASE I, II AND III; GENERAL TRANSCRIPTION FACTORS, THE MEDIATOR COMPLEX; MESSENGER RNA PROCESSING: CAPPING, SPLICING AND POLYADENYLATION; THE SPLICEOSOME, ALTERNATIVE SPLICING.
TRANSLATION: THE GENETIC CODE; STRUCTURE AND FUNCTION OF TRNA, AMINOACYL-TRNA SYNTHETASES, THE RIBOSOME, INITIATION, ELONGATION AND TERMINATION OF TRANSLATION.
REGULATION OF GENE EXPRESSION: TRANSCRIPTIONAL ACTIVATORS; SIGNAL INTEGRATION AND COMBINATORIAL CONTROL, SIGNAL TRANSDUCTION AND CONTROL OF TRANSCRIPTIONAL REGULATORS; GENE SILENCING, RNA INTERFERENCE; SYNTHESIS AND FUNCTION OF MIRNAS.
MOLECULAR BIOLOGY TECHNOLOGY AND GENE EXPRESSION ANALYSIS: PURIFICATION OF BIOLOGICVAL MACROMOLECULES; ELECTROPHORESIS, RESTRICTION ENZYMES; EMSA; FOOTPRINTING; PCR, RT-PCR, DNA SEQUENCING, MOLECULAR DIAGNOSTICS AND CLINICAL IMPLICATIONS; HIGH THROUGHPUT TECHNOLOGIES.

MODULE C: PRINCIPLES OF LABORATORY MEDICINE; BLOOD WITHDRAWALS FOR HEMATOCHEMISTRY; ANEMIA; INTERPRETATION OF ROUTINE ANALYSIS, COMPLETE BLOOD COUNT, INTERPRETATION AND MARKERS OF LABORATORY MEDICINE DATA IN THE FOLLOWING PATHOLOGIES: ISCHEMIC HEART DISEASE, DIABETES, DEMYELINATING DISEASES, MYOPATHY, ENDOTHELIAL DYSFUNCTION; HEPATITIS; KIDNEY FUNCTIONALITY; MARKERS OF INFLAMMATION.

Teaching Methods
THE COURSE IS DIVIDED INTO TWO MODULES AND PROVIDES 143 HOURS OF ASSISTED LEARNING (13 CFU) ALLOCATED BETWEEN THE TEACHING HOURS IN THE FORM OF CLASSROOM LECTURES AND HOURS OF SUPPLEMENTARY ACTIVITIES (ADI) CONSIST OF SEMINARS, LABORATORY EXERCISES, PRACTICE EXAMS, CORRECTIONS, STUDY GROUPS FOR THE ANALYSIS AND ELABORATION OF SCIENTIFIC PUBLICATIONS
Verification of learning
THE LEVEL OF ACHIEVEMENT OF THE OBJECTIVES OF TEACHING IS CERTIFIED BY PASSING AN EXAM WITH EVALUATION OF THIRTY. VERIFICATION INVOLVES AN ORAL TEST
THE ORAL TEST IS INTENDED TO ASCERTAIN THE LEVEL OF KNOWLEDGE OF THEORETICAL CONTENTS; THE LEVEL OF EXPERTISE ACHIEVED IN THE EXHIBITION SUCH AS LANGUAGE PROPERTY, LOGICAL - DEDUCTIVE AND SYNTHESIS SKILLS. THE MINIMUM VOTE FOR PASSING THE EXAM IS 18/30. PRAISE MAY BE ATTRIBUTED TO STUDENTS WHO DEMONSTRATE THAT THEY ARE ABLE TO APPLY THEIR OWN KNOWLEDGE AND SKILLS ACQUIRED IN CONTEXTS OTHER THAN THOSE PROPOSED IN LESSONS.
Texts
BAYNES AND DOMINICZAK, BIOCHIMICA MEDICA, ED. ELSEVIER
LEHNINGER, NELSON & COX, PRINCIPI DI BIOCHIMICA, ED. ZANICHELLI
MATHEWS, VAN HOLD, AHEREN, BIOCHIMICA, ED. AMBROSIANA
STRYER, BIOCHIMICA, ED. ZANICHELLI
VOET & VOET, BIOCHIMICA, ED. ZANICHELLI
CAMPBELL & FARRELL, BIOCHIMICA, ED. EDISES
SILIPRANDI-TETTAMANTI. BIOCHIMICA MEDICA – STRUTTURALE, METABOLICA E FUNZIONALE
IV EDIZIONE. PICCIN
J.D. WATSON ET AL, BIOLOGIA MOLECOLARE DEL GENE, SESTA EDIZIONE CASA ED. ZANICHELLI
LEWIN ET AL., CELLULE, CASA ED. ZANICHELLI
ALBERTS ET AL., “BIOLOGIA MOLECOLARE DELLA CELLULA”, V EDIZIONE, CASA ED. ZANICHELLI
WEAVER, BIOLOGIA MOLECOLARE, CASA ED. MCGRAW-HILL
MEDICINA DI LABORATORIO - LOGICA E PATOLOGIA CLINICA
AUTORE: ANTONOZZI - GULLETTA, EDITORE: PICCIN, EDIZIONE: II 2014, VOLUME:UNICO
LECTURES SLIDES
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